[ IDENTIFICATION DES FACTEURS PHYSIOLOGIQUES CONTROLANT LE POLYPENISME PHASAIRE CHEZ LE CRIQUET MIGRATEUR ]
Volume 20, Issue 1, January 2016, Pages 242–252
Amel Ben Hamouda1, Mohamed Ammar2, Arnold De Loof3, Abderrahmen Bouain4, and Mohamed Habib Ben Hamouda5
1 Laboratoire des Sciences Biologiques et Protection des Plantes, Institut Supérieur Agronomique de Chott-Mariem, Sousse, Tunisia
2 Laboratoire des Bio-agresseurs et Protection Intégrée en Agriculture, Institut National Agronomique de Tunis, Tunisia
3 Laboratory for Developmental Physiology, Genomics and Proteomics, Zoological Institute, Leuven, Belgium
4 Laboratoire des Sciences de la Vie, Faculté des Sciences de Sfax, Tunisia
5 Laboratoire des Bio-agresseurs et Protection Intégrée en Agriculture, Institut National Agronomique de Tunis, Tunisia
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The gregarization in the migratory locust, Locusta migratoria and its trans-generational characteristic were controlled by the maternal gregarizing factor which secreted in the egg pod foam by the female's accessory glands. The comparison by SDS-PAGE of the contents of these glands showed the presence of a supplementary proteic band specific of gregarious phase with a molecular weight 30 KDa, absent in the solitary phase. The synthesis of the maternal gregarization factor was determined in gregarious accessory glands under the control of another factor, obtained by SDS-PAGE in the haemolymph of gregarious females. The trans-generational specificity of the maternal gregarizing factor was revealed in the presence in gregarious oocytes of a protein band of the same molecular weight (30 KDa) as that observed in the accessory glands. Analysis of gregarious egg pod foam by Thin Layer Chromatography showed the presence of a supplementary spot. No solitary phase specific spots were obtained in solitary foam matter. In gregarious eggs, a proteic band of molecular weight 263 KDa exists throughout the first 6 days of embryonic life. It disappeared since the first day in solitary phase. Gregarious hatchlings also showed a difference in the proteins content of their haemolymph; a proteic band of molecular weight of 66 KDa was observed in gregarious phase, absent in solitary one.These results prove that gregarious egg pod foam represents a physiological transporter of the maternal gregarizing factor from the female accessory glands to oocytes.
Author Keywords: Maternal gregarizing factor, phase polymorphism, Locusta migratoria.
Volume 20, Issue 1, January 2016, Pages 242–252
Amel Ben Hamouda1, Mohamed Ammar2, Arnold De Loof3, Abderrahmen Bouain4, and Mohamed Habib Ben Hamouda5
1 Laboratoire des Sciences Biologiques et Protection des Plantes, Institut Supérieur Agronomique de Chott-Mariem, Sousse, Tunisia
2 Laboratoire des Bio-agresseurs et Protection Intégrée en Agriculture, Institut National Agronomique de Tunis, Tunisia
3 Laboratory for Developmental Physiology, Genomics and Proteomics, Zoological Institute, Leuven, Belgium
4 Laboratoire des Sciences de la Vie, Faculté des Sciences de Sfax, Tunisia
5 Laboratoire des Bio-agresseurs et Protection Intégrée en Agriculture, Institut National Agronomique de Tunis, Tunisia
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The gregarization in the migratory locust, Locusta migratoria and its trans-generational characteristic were controlled by the maternal gregarizing factor which secreted in the egg pod foam by the female's accessory glands. The comparison by SDS-PAGE of the contents of these glands showed the presence of a supplementary proteic band specific of gregarious phase with a molecular weight 30 KDa, absent in the solitary phase. The synthesis of the maternal gregarization factor was determined in gregarious accessory glands under the control of another factor, obtained by SDS-PAGE in the haemolymph of gregarious females. The trans-generational specificity of the maternal gregarizing factor was revealed in the presence in gregarious oocytes of a protein band of the same molecular weight (30 KDa) as that observed in the accessory glands. Analysis of gregarious egg pod foam by Thin Layer Chromatography showed the presence of a supplementary spot. No solitary phase specific spots were obtained in solitary foam matter. In gregarious eggs, a proteic band of molecular weight 263 KDa exists throughout the first 6 days of embryonic life. It disappeared since the first day in solitary phase. Gregarious hatchlings also showed a difference in the proteins content of their haemolymph; a proteic band of molecular weight of 66 KDa was observed in gregarious phase, absent in solitary one.These results prove that gregarious egg pod foam represents a physiological transporter of the maternal gregarizing factor from the female accessory glands to oocytes.
Author Keywords: Maternal gregarizing factor, phase polymorphism, Locusta migratoria.
Abstract: (french)
La grégarisation chez le criquet migrateur, Locusta migratoria et son attribut trans-générationnel sont contrôlés par la substance femelle de grégarisation (SFG) sécrétée par les glandes accessoires femelles au niveau de la matière spumeuse. La comparaison du contenu de ces glandes par la technique d'électrophorèse sur polyacrylamide (SDS-PAGE) a montré la présence d'une bande protéique différentielle (30 KDa) spécifique de la phase grégaire et absente chez la phase solitaire. La synthèse de cette substance, déterminée au niveau des glandes accessoires grégaires est sous le contrôle d'un autre facteur, décelé par SDS-PAGE au niveau de l'hémolymphe des femelles grégaires. La spécificité trans-générationnelle de la SFG se révèle par la présence au niveau des ovocytes grégaires de la même bande protéique (30 KDa) observée au niveau des glandes accessoires. L'analyse de la matière spumeuse grégaire par chromatographie sur couche mine a montrée la présence d'un spot supplémentaire, absent au niveau de la matière spumeuse solitaire. Au niveau des œufs grégaires, une bande protéique (263 KDa) existe tout au long des 6 premiers jours de la vie embryonnaire, mais qui disparait après la ponte au niveau des œufs solitaires. Les larves du premier stade grégaires présentent aussi une différence au niveau des protéines de l'hémolymphe avec une bande protéique de 66 KDa chez les larves grégaires, absente chez leurs congénères solitaires. Ainsi, la matière spumeuse grégaire joue le rôle de transporteur physiologique de la SFG des glandes accessoires vers les ovocytes.
Author Keywords: Substance femelle de grégarisation, polymorphisme phasaire, Locusta migratoria.
How to Cite this Article
Amel Ben Hamouda, Mohamed Ammar, Arnold De Loof, Abderrahmen Bouain, and Mohamed Habib Ben Hamouda, “IDENTIFICATION OF PHYSIOLOGICAL FACTORS RESPONSIBLE FOR PHASE POLYMORPHISM OF MIGRATORY LOCUST,” International Journal of Innovation and Scientific Research, vol. 20, no. 1, pp. 242–252, January 2016.
