Volume 10, Issue 2, October 2014, Pages 570–575
James Ronald BAYOÏ1, Darman Roger DJOULDE2, Patrice BOGNE KAMGA3, Maximilienne NYEGUE4, Steve VOUNDI OLUGU5, Daoudou BAKARY6, and Francois-Xavier ETOA7
1 Département des Sciences Biologiques, Université de Maroua, Faculté des Sciences, BP 814, Maroua, Cameroon
2 Département d'Agriculture, Élevage et produits dérivés (AGEPD) Université de Maroua, Institut Supérieur du Sahel (ISS), BP 46, Maroua, Cameroon
3 Laboratory of Microbiology, Department of Food Science and Nutrition, University of Ngaoundere, BP 455 Ngaoundere, Cameroon
4 Laboratory of Microbiology, Department of Microbiology, University of Yaounde I, BP 812 Yaounde, Cameroon
5 Laboratory of Microbiology, Department of Microbiology, University of Yaounde I, BP 812 Yaounde, Cameroon
6 Département des Sciences Biologiques, Université de Maroua, Faculté des Sciences, BP 814, Maroua, Cameroon
7 Département de Microbiologie, Université de Yaoundé I, Faculté des Sciences, BP 812, Yaoundé, Cameroon
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This work has been led in view to find the influence of sublethal heat (45°C, 50°C, 60°C) on acid resistance of B. subtilis NCTC 3610 and B. stearothermophilus CNCH 5781 spores. Firstly, we have submitted Bacillus spores to 0.4% acetic acid pH 4.5 during the times of 1, 2 and 3 hours. Then another spores group were preheated at various sublethal temperatures, before be treated with acetic acid. The effect of acetic acid before and after preheat was evaluated by the culture of treated spores on agar medium and the number of colony obtained was compared with that of control culture (neither treated with heat nor acid) and control A culture (only treated with acid). We found that acetic acid was effective on the twice Bacillus spores with more effect on B. stearothermophilus CNCH 5781 spores. Furthermore we have noticed a significant increasing in percentages of recovery of colonies obtained from preheated and acid treated spores compared to those of control and control A cultures. This increase of recovery percentages could be demonstrated the manifestation of a "heat-induced acid resistance" phenomenon. Yet, this phenomenon was more accentuated for preheatings at 50 and 60°C during 3 and 2 hours, respectively for B. subtilis and B. stearothermophilus spores. This study suggest that sublethal heats could be play major role in protection of microorganisms to chemicals
Author Keywords: sublethal heats, acid resistance, spores, Bacillus.
James Ronald BAYOÏ1, Darman Roger DJOULDE2, Patrice BOGNE KAMGA3, Maximilienne NYEGUE4, Steve VOUNDI OLUGU5, Daoudou BAKARY6, and Francois-Xavier ETOA7
1 Département des Sciences Biologiques, Université de Maroua, Faculté des Sciences, BP 814, Maroua, Cameroon
2 Département d'Agriculture, Élevage et produits dérivés (AGEPD) Université de Maroua, Institut Supérieur du Sahel (ISS), BP 46, Maroua, Cameroon
3 Laboratory of Microbiology, Department of Food Science and Nutrition, University of Ngaoundere, BP 455 Ngaoundere, Cameroon
4 Laboratory of Microbiology, Department of Microbiology, University of Yaounde I, BP 812 Yaounde, Cameroon
5 Laboratory of Microbiology, Department of Microbiology, University of Yaounde I, BP 812 Yaounde, Cameroon
6 Département des Sciences Biologiques, Université de Maroua, Faculté des Sciences, BP 814, Maroua, Cameroon
7 Département de Microbiologie, Université de Yaoundé I, Faculté des Sciences, BP 812, Yaoundé, Cameroon
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This work has been led in view to find the influence of sublethal heat (45°C, 50°C, 60°C) on acid resistance of B. subtilis NCTC 3610 and B. stearothermophilus CNCH 5781 spores. Firstly, we have submitted Bacillus spores to 0.4% acetic acid pH 4.5 during the times of 1, 2 and 3 hours. Then another spores group were preheated at various sublethal temperatures, before be treated with acetic acid. The effect of acetic acid before and after preheat was evaluated by the culture of treated spores on agar medium and the number of colony obtained was compared with that of control culture (neither treated with heat nor acid) and control A culture (only treated with acid). We found that acetic acid was effective on the twice Bacillus spores with more effect on B. stearothermophilus CNCH 5781 spores. Furthermore we have noticed a significant increasing in percentages of recovery of colonies obtained from preheated and acid treated spores compared to those of control and control A cultures. This increase of recovery percentages could be demonstrated the manifestation of a "heat-induced acid resistance" phenomenon. Yet, this phenomenon was more accentuated for preheatings at 50 and 60°C during 3 and 2 hours, respectively for B. subtilis and B. stearothermophilus spores. This study suggest that sublethal heats could be play major role in protection of microorganisms to chemicals
Author Keywords: sublethal heats, acid resistance, spores, Bacillus.
How to Cite this Article
James Ronald BAYOÏ, Darman Roger DJOULDE, Patrice BOGNE KAMGA, Maximilienne NYEGUE, Steve VOUNDI OLUGU, Daoudou BAKARY, and Francois-Xavier ETOA, “Activity of Acetic Acid on Bacillus stearothermophilus and Bacillus subtilis Spores after Sublethal Heat Pretreatments,” International Journal of Innovation and Scientific Research, vol. 10, no. 2, pp. 570–575, October 2014.
