Volume 5, Issue 1, July 2014, Pages 1–8
Camara Ce1, Linyuan Zhou2, Dong Yu3, Yaowu Zhao4, and Nianhong Yang5
1 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
2 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
3 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
4 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
5 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: This study aims to investigate ovariectomy effect on serum osteocalcin levels and bone mineral density in rats. Methods: Forty five female Sprague Dawley rats were divided into 3 groups (n = 15 rats per group): sham-operated control, Ovariectomized, and Ovariectomized plus estrogen. After 3 months of experiment, rats were killed by decapitation after a 12 hour fast, obtained serum from rats' blood sample was kept at –80°C. For all groups of rats, serum osteocalcin levels were measured by ELISA. The rats' left femoral bone mineral density was measured by dual energy X-ray absorptiometry. Weights and lengths of the left femur were measured with electronic balance and Vernier caliper respectively.
Results: This study revealed an increase in serum osteocalcin levels in ovariectomized rats and in ovariectomized plus estrogen rats, alongside decrease in left distal femur bone mineral density in both groups was observed when compared to the control. The mean values of both serum osteocalcin and bone mineral density between the ovariectomized rats and the control were statistically significant. Estrogen administration in ovariectomized rats showed no significant changes in both serum osteocalcin levels and bone mineral density.
Conclusion: This study concludes that estrogen-deficiency by ovariectomy induces an increase in bone turnover with higher serum osteocalcin levels in ovariectomized rats and that the combination of serum osteocalcin levels and bone mineral density measurement may be a better predictor of the fracture risk.
Author Keywords: estrogen, bone turnover, post menopause, osteoporosis, serum osteocalcin.
Camara Ce1, Linyuan Zhou2, Dong Yu3, Yaowu Zhao4, and Nianhong Yang5
1 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
2 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
3 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
4 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
5 Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objective: This study aims to investigate ovariectomy effect on serum osteocalcin levels and bone mineral density in rats. Methods: Forty five female Sprague Dawley rats were divided into 3 groups (n = 15 rats per group): sham-operated control, Ovariectomized, and Ovariectomized plus estrogen. After 3 months of experiment, rats were killed by decapitation after a 12 hour fast, obtained serum from rats' blood sample was kept at –80°C. For all groups of rats, serum osteocalcin levels were measured by ELISA. The rats' left femoral bone mineral density was measured by dual energy X-ray absorptiometry. Weights and lengths of the left femur were measured with electronic balance and Vernier caliper respectively.
Results: This study revealed an increase in serum osteocalcin levels in ovariectomized rats and in ovariectomized plus estrogen rats, alongside decrease in left distal femur bone mineral density in both groups was observed when compared to the control. The mean values of both serum osteocalcin and bone mineral density between the ovariectomized rats and the control were statistically significant. Estrogen administration in ovariectomized rats showed no significant changes in both serum osteocalcin levels and bone mineral density.
Conclusion: This study concludes that estrogen-deficiency by ovariectomy induces an increase in bone turnover with higher serum osteocalcin levels in ovariectomized rats and that the combination of serum osteocalcin levels and bone mineral density measurement may be a better predictor of the fracture risk.
Author Keywords: estrogen, bone turnover, post menopause, osteoporosis, serum osteocalcin.
How to Cite this Article
Camara Ce, Linyuan Zhou, Dong Yu, Yaowu Zhao, and Nianhong Yang, “Serum osteocalcin levels and bone mineral density in ovariectomized rats,” International Journal of Innovation and Scientific Research, vol. 5, no. 1, pp. 1–8, July 2014.
