The research was assessed to evaluate the efficacy of crude extract of Psidium guajava against selected bacterial strains. The extract of Psidium guajava leaves was obtained by three different methods and the inhibition zones obtained through disc diffusion method. In this study, P. guajava extract displayed some degree of antimicrobial activity and presented compound. It was revealed from the results that Psidium guajava leaf extracts shows different degree of inhibition against different microorganisms. This investigation suggests the Psidium guajava to be a good antimicrobial agent against various pathogenic agents. Further investigation is required in this regards that can replace the plant with a very beneficial antimicrobial medicine and enhance its effects.