In this study we were interested in assessing the impact of ZnO nanoparticles and their effects on physiological and biochemical parameters of durum wheat (Triticum turgidum ssp. durum). The impact of ZnO is evaluated in three wheat varieties (Boussallem, Gtadur and Ouarsenis) varieties are represented by V1, V3 V2et through a range of physiological and biochemical parameters made in the laboratory. The selected varieties were exposed to increasing concentrations of ZnO nanoparticles (0.01 mg / ml, 0.05mg / ml, 0.1mg / ml, and 0.5mg / ml). Preliminary results show that the presence of ZnO-based NPs can have a stimulating effect as inhibitor concentration and the effect studied parameter: 1. Effect concentration and a reduction of the levels of chlorophyll (chlo a, chlo b) induced by exposure to low concentrations. 2. The presence of the nanoparticles resulted in oxidative stress resulting in the accumulation of Lipoperoxydes (MDA) recorded in all varieties studied after exposure to different concentrations. 3. Furthermore varietal effect contributed much of the remaining variation in parameters related to ROS (carotenoids, APx and CAT).