Medium and large-sized mammals worldwide are facing a critical survival challenge due to human activities. In Mont Sangbé National Park (MSNP) in western Côte d’Ivoire, medium and large-sized mammals are frequently sighted in the natural salt licks of the savanna zone, yet there is limited knowledge regarding this fauna. This study seeks to assess the diversity of mammals in the park’s natural salt licks and evaluate potential threats posed by human activities. We employed camera trapping and reconnaissance walks at these natural salt licks to collect data. During reconnaissance walks, 58 signs of medium and large mammals were observed, including feces, footprints, feeding remnants et burrows. A total of 19 species of medium and large-sized mammals, distributed across six (06) orders and 11 families, were identified. Among these species, six (06) were directly observed during reconnaissance walks, and 16 were observed with camera traps. Fifteen of the observed species are classified as least concerned, one is near threatened and three are vulnerable according to the International Union for Conservation of Nature. Furthermore, we documented evidence of human activities near the salt licks, underscoring significant anthropogenic threats to these species. Comprehensive research covering all MSNP natural salt licks and accounting for seasonal variations is essential for gaining a deeper understanding of the ecology of mammals frequenting these areas and ensuring their preservation.