[ L'étude de l'effet insecticide de l'huile essentielle de Syzygium aromaticum L. contre les larves de Tuta absoluta ]
Volume 20, Issue 1, January 2016, Pages 188–194
Adil Benchouikh1, Tarik Allam2, Abderahim Kribii3, and Khadija Ounine4
1 Laboratoire de biologie et santé, équipe de microbiologie appliquée, Faculté des sciences, Université Ibn Tofaïl., Maroc
2 Laboratoire de Biologie et Santé, Equipe de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofail, B.P. 242. Kénitra, Maroc
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The tomato leafminer, Tuta absoluta (Meyrick), is a major pest of tomatoes in Morocco. Since its spread in the 1970s, chemical control was the main method of control. However, reduced effectiveness of some of the recommended insecticides has been observed since the 1980s. This study aims to propose alternative solutions based on the use of natural products "bioinsecticide" to fight against the tomato leafminer, Tuta absoluta (Meyrick), which is considered a serious threat to tomato production in Morocco and worldwide.
To achieve this objective, we evaluated the toxicity of the essential oil of Syzygium aromaticum L. buds against larvae of Tuta absoluta. Bioassays were performed in the laboratory in petri dishes 9 cm in diameter, at an average temperature of 26 ± 2 ° C and a relative humidity of 60 to 70%. The determination the lethal dose 50 (LD50) of this essential oil has been made via an analysis by probit method using the software BioStat Pro 5.9.8. The values of LD50 and LD100 are respectively 0.0047 and 0,0111μl/cm² calculated after fourths hours of exposure confirms the high degree of toxicity of the essential oil of Syzygium aromaticum L. buds on filter paper against the larvae of Tuta absoluta.
Author Keywords: Syzygium aromaticum L., Tuta absulota, Bioinsecticide, insect repellent activity.
Volume 20, Issue 1, January 2016, Pages 188–194
Adil Benchouikh1, Tarik Allam2, Abderahim Kribii3, and Khadija Ounine4
1 Laboratoire de biologie et santé, équipe de microbiologie appliquée, Faculté des sciences, Université Ibn Tofaïl., Maroc
2 Laboratoire de Biologie et Santé, Equipe de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofail, B.P. 242. Kénitra, Maroc
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The tomato leafminer, Tuta absoluta (Meyrick), is a major pest of tomatoes in Morocco. Since its spread in the 1970s, chemical control was the main method of control. However, reduced effectiveness of some of the recommended insecticides has been observed since the 1980s. This study aims to propose alternative solutions based on the use of natural products "bioinsecticide" to fight against the tomato leafminer, Tuta absoluta (Meyrick), which is considered a serious threat to tomato production in Morocco and worldwide.
To achieve this objective, we evaluated the toxicity of the essential oil of Syzygium aromaticum L. buds against larvae of Tuta absoluta. Bioassays were performed in the laboratory in petri dishes 9 cm in diameter, at an average temperature of 26 ± 2 ° C and a relative humidity of 60 to 70%. The determination the lethal dose 50 (LD50) of this essential oil has been made via an analysis by probit method using the software BioStat Pro 5.9.8. The values of LD50 and LD100 are respectively 0.0047 and 0,0111μl/cm² calculated after fourths hours of exposure confirms the high degree of toxicity of the essential oil of Syzygium aromaticum L. buds on filter paper against the larvae of Tuta absoluta.
Author Keywords: Syzygium aromaticum L., Tuta absulota, Bioinsecticide, insect repellent activity.
Abstract: (french)
La mineuse de la tomate, Tuta absoluta (Meyrick), est l'un des principaux ravageurs de la tomate au Maroc. Depuis sa dispersion dans les années 1970, la lutte chimique a été la principale méthode de contrôle. Cependant, l'efficacité réduite de certains des insecticides recommandés a été observé depuis les années 1980. La présente étude a pour objectif de proposer des solutions alternatives basées sur l'utilisation des produits naturels «bioinsecticide», afin de lutter contre la mineuse de la tomate, Tuta absoluta (Meyrick) qui est considérée comme une menace sérieuse pour la production de la tomate au Maroc et au monde entier.
Pour répondre à cet objectif, nous avons évalué la toxicité de l'huile essentielle des clous de Syzygium aromaticum L. sur des larves de Tuta absoluta. Les bio-essais ont été effectués au laboratoire dans des boîtes de Pétri de 9 cm de diamètre, à une température moyenne de 26 ± 2 °C et une humidité relative de 60 à 70%. La détermination de la dose létale 50 (DL50) de cette huile essentielle a été faite via une analyse par la méthode de probits à l'aide du logiciel BioStat Pro 5.9.8 .La plus forte dose de l'huile essentielle des clous de Syzygium aromaticum L. occasionne une mortalité totale (100%) des larves est de 0.0111μl/cm². La DL50 est de 0,0047μl/cm² calculée après quartes heures d'exposition confirme le degré élevé de toxicité de cette huile essentielle sur papier filtre vis-à-vis des larves de Tuta absoluta.
Author Keywords: Syzygium aromaticum L., Tuta absulota, Bioinsecticide, activité insectifuge.
How to Cite this Article
Adil Benchouikh, Tarik Allam, Abderahim Kribii, and Khadija Ounine, “The study of the insecticide effect of the essential oil of Syzygium aromaticum L. against larvae of Tuta absoluta,” International Journal of Innovation and Scientific Research, vol. 20, no. 1, pp. 188–194, January 2016.
