Volume 18, Issue 2, October 2015, Pages 407–416
Zineb Mammad1, Tormal Djassinra2, Abderahim Kribii3, and Khadija Ounine4
1 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
2 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Maroc
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
Original language: French
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Salvadora Persica is a plant known for its medicinal properties whose variety of pharmacological activities have been reported by many studies. Volatile extracts of Salvadora persica are obtained either by hydrodistilation or by using a Soxhlet apparatus from crushed roots by using hexane as solvent. The obtained result by hexane extraction 2.5% has given better results than the one got by steam distillation 0.05%. Thus, the extracts obtained have been analyzed by coupling (GC-MS). The following products are among the many products identified: The Eugenol, the Asarone the Borneol the Carvacrol, vanillin, the Cuminal, Camphor, Acetamide the n-benzyl, the benzyl Isocianate and the oily acids Oleic and palmitic that represents the majority of the products extracted.
The essential oil of action Persica - on Streptococcus faecalis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa by the method aromatogram- shows that these bacteria are inhibited. Therefore, essential oil Miswak generated a zone of inhibition of 40, 25 and 15mm respectively on Streptococcus faecalis, Escherichia coli and Staphylococcus aureus. Meanwhile, it did not influence Pseudomonas aeruginosa.
The antimicrobial activity of the essential oils has been evaluated by determining the minimum inhibitory concentration (MIC). Comparing the antibacterial effect with antibiotics, it was observed that the Miswak has a noticeable antibacterial power. It may, then, be an excellent oral hygiene agent.
Author Keywords: Salvadora Persica, extracts, essential oil, Soxhlet, GC-MS, Streptococcus faecalis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa MC1, Aromatogram.
Zineb Mammad1, Tormal Djassinra2, Abderahim Kribii3, and Khadija Ounine4
1 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
2 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Maroc
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
Original language: French
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Salvadora Persica is a plant known for its medicinal properties whose variety of pharmacological activities have been reported by many studies. Volatile extracts of Salvadora persica are obtained either by hydrodistilation or by using a Soxhlet apparatus from crushed roots by using hexane as solvent. The obtained result by hexane extraction 2.5% has given better results than the one got by steam distillation 0.05%. Thus, the extracts obtained have been analyzed by coupling (GC-MS). The following products are among the many products identified: The Eugenol, the Asarone the Borneol the Carvacrol, vanillin, the Cuminal, Camphor, Acetamide the n-benzyl, the benzyl Isocianate and the oily acids Oleic and palmitic that represents the majority of the products extracted.
The essential oil of action Persica - on Streptococcus faecalis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa by the method aromatogram- shows that these bacteria are inhibited. Therefore, essential oil Miswak generated a zone of inhibition of 40, 25 and 15mm respectively on Streptococcus faecalis, Escherichia coli and Staphylococcus aureus. Meanwhile, it did not influence Pseudomonas aeruginosa.
The antimicrobial activity of the essential oils has been evaluated by determining the minimum inhibitory concentration (MIC). Comparing the antibacterial effect with antibiotics, it was observed that the Miswak has a noticeable antibacterial power. It may, then, be an excellent oral hygiene agent.
Author Keywords: Salvadora Persica, extracts, essential oil, Soxhlet, GC-MS, Streptococcus faecalis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa MC1, Aromatogram.
Abstract: (french)
Salvadora persica est une plante connue pour ses vertus médicinales dont de nombreuses études ont rapporté diverses activités pharmacologiques.
Les extraits volatils de Salvadora persica sont obtenus soit par hydrodistilation, soit à l'aide d'un appareil de Soxhlet à partir des racines broyées en utilisant l'hexane comme solvant. Le rendement obtenu par extraction à l'hexane 2.5% est beaucoup plus significatif que celui obtenu par hydrodistillation 0.05%. Les extraits ainsi obtenus sont analysés par couplage (CG-SM). Les produits cités ci-suit sont parmi les nombreux produits identifiées :
l'Eugenol, l'Asarone, le Borneol, le Carvacrol, la Vaniline, le Cuminal, le Camphre, l'Acetamide n-benzyl, l'Isocianate de benzyl et les acides gras Oleique et palmitique qui representent les produits majoritaire des extraits. L'action d'huile essentielle de Salvadora Persica sur Streptococus faecalis, Escherichia Coli, Staphylococcus aureus et Pseudomonas aeruginosa par la méthode d'aromatogramme montre que ces bactéries sont inhibées. En effet l'huile essentielle de Miswak a engendré une zone d'inhibition de 40, 25 et 15mm respectivement sur Streptococcus faecalis, Escherichia Coli et Staphylococcus aureus. Alors qu'elle n'a pas donné d'effet sur Pseudomonas aeruginosa.
L'activité antimicrobienne des huiles essentielles a été évaluée par la détermination des concentrations minimales inhibitrices (CMI). En comparant l'effet antibactérien avec les antibiotiques, on observe que le Miswak possède un pouvoir antibactérien notable. Il peut, alors, constituer un excellent agent d'hygiène buccale.
Author Keywords: Salvadora persica, huiles essentielles, Soxhlet, GC-MS, Streptocoque faecalis, Escherichia Coli, Staphylococcus aureus, Pseudomonas aeruginosa MC1, Aromatogramme.
How to Cite this Article
Zineb Mammad, Tormal Djassinra, Abderahim Kribii, and Khadija Ounine, “Evaluation de l'activité antibactérienne du Salvadora Persicaet leur caractérisation chimique,” International Journal of Innovation and Scientific Research, vol. 18, no. 2, pp. 407–416, October 2015.
