Volume 16, Issue 2, July 2015, Pages 425–432
Fatima Zahra ESSADIK1, Sara HAIDA2, Abderahim Kribii3, Abdelaziz Ramadane KRIBII4, Khadija Ounine5, and Amar HABSAOUI6
1 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
2 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
5 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
6 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The essential oil isolated from western Moroccan Citrus aurantium L. var. amara peel, by hydrodistillation, is analyzed by Gas Chromatography-mass spectrometry (GC-MS). Fifteen compounds representing 99.86% of total oil are identified. The predominant components in the essential oil are limonene (90.90%), linalool (2.52%), myrcene (1.51%) and β-pinene (1.41%).
Being both dietary and biologically active compounds, flavonoids have attracted much attention of investigations as potent species capable of affecting various biological processes in living organisms. The total flavonoids contents of aqueous and methanolic extracts of bitter orange's peel were found to be 1.43 and 14.82 mg Equivalent Quercetin/g respectively.
Furthermore, the antioxidant activity of aqueous and methanolic extracts of bitter orange's peel is measured using free radical scavenging method with DPPH•. In addition, a preliminary study of the antibacterial activity of extracts of bitter orange's peel is tested against three bacterial stains: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
Author Keywords: Bitter orange, DPPH•, flavonoids, hydrodistillation, free radical scavenging.
Fatima Zahra ESSADIK1, Sara HAIDA2, Abderahim Kribii3, Abdelaziz Ramadane KRIBII4, Khadija Ounine5, and Amar HABSAOUI6
1 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
2 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
3 Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco
4 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
5 Laboratoire de Microbiologie Appliquée, Faculté des Sciences, Université Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco
6 Department of Chemistry, Faculty of Sciences, University Ibn Tofaїl, Box 133, 14000, Kenitra, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The essential oil isolated from western Moroccan Citrus aurantium L. var. amara peel, by hydrodistillation, is analyzed by Gas Chromatography-mass spectrometry (GC-MS). Fifteen compounds representing 99.86% of total oil are identified. The predominant components in the essential oil are limonene (90.90%), linalool (2.52%), myrcene (1.51%) and β-pinene (1.41%).
Being both dietary and biologically active compounds, flavonoids have attracted much attention of investigations as potent species capable of affecting various biological processes in living organisms. The total flavonoids contents of aqueous and methanolic extracts of bitter orange's peel were found to be 1.43 and 14.82 mg Equivalent Quercetin/g respectively.
Furthermore, the antioxidant activity of aqueous and methanolic extracts of bitter orange's peel is measured using free radical scavenging method with DPPH•. In addition, a preliminary study of the antibacterial activity of extracts of bitter orange's peel is tested against three bacterial stains: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
Author Keywords: Bitter orange, DPPH•, flavonoids, hydrodistillation, free radical scavenging.
How to Cite this Article
Fatima Zahra ESSADIK, Sara HAIDA, Abderahim Kribii, Abdelaziz Ramadane KRIBII, Khadija Ounine, and Amar HABSAOUI, “Antioxidant activity of Citrus aurantium L. var. amara Peel from western of Morocco, identification of volatile compounds of its essential oil by GC-MS and a preliminary study of their antibacterial activity,” International Journal of Innovation and Scientific Research, vol. 16, no. 2, pp. 425–432, July 2015.
