|
Twitter
|
Facebook
|
Google+
|
VKontakte
|
LinkedIn
|
 
 
International Journal of Innovation and Scientific Research
ISSN: 2351-8014
 
 
Sunday 13 October 2019

About IJISR

News

Submission

Downloads

Archives

Custom Search

Contact

Connect with IJISR

   
 
 
 

Regulation of Callogenesis using Cotyledon and Hypocotyls explants of Cassia senna


Volume 14, Issue 1, March 2015, Pages 49–54

 Regulation of Callogenesis using Cotyledon and Hypocotyls explants of Cassia senna

Khadiga Gaafar Abd Elaleem, Magda Mohamed Ahmed, and Afarah Adiel Elshiekh Alhassan

Original language: English

Received 8 February 2015

Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


The present study was carried out to detect the effect of two Auxin namely dichlorophenoxy acetic acid (2, 4-D) and naphthalene acetic acid (NAA) on callus induction from cotyledon and hypocotyledon explants of cassia senna sp. Two explant from in vitro cassia micro plant were used for Callus induction on Murashige and Skoog media (MS) media, as basal media (control) or supplemented with different concentration of 2, 4, - D dichlorophenoxy acetic acid (2,4,D) and naphthalene acetic acid (NAA). On MS basal media (without growth regulators) no callusing obtained. Cotyledon and hypocotyls explants obtained callusing on MS media supplemented with NAA and 2, 4-D at all concentration . Callus growth percentage of cotyledonary leaf segments is varied from 60% to 100%, while the callus growth percentage of hypocotyl segment explant is varied from 75%-to 100%. Highest percentage of callus (100%) was observed in medium supplemented with 2.5 mg/l of the both NAA and 2.4-D. The both explant induce compact callus with all treatment .For callus color , cotyledon explant produced yellowish callus with the both auxin , on the other hand hypocotyls explant produced green to light green callus The highest callus degree (2.55±0.11) was obtained in MS media supplemented with 2.5 mg/l NAA by cotyledon explant , followed by (2.45± 0.15) induced in MS media fortified with 2.5 mg/l NAA(the same treatment) from hypocotyl explant, during same period of time(14 day).

Author Keywords: callus, growth regulators, cotyledon explant, hypocotyl segment, auxin.


How to Cite this Article


Khadiga Gaafar Abd Elaleem, Magda Mohamed Ahmed, and Afarah Adiel Elshiekh Alhassan, “Regulation of Callogenesis using Cotyledon and Hypocotyls explants of Cassia senna,” International Journal of Innovation and Scientific Research, vol. 14, no. 1, pp. 49–54, March 2015.