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International Journal of Innovation and Scientific Research
ISSN: 2351-8014
 
 
Saturday 24 August 2019

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Quantitative Determination of Sugar Levels in Natural Plants of Cactus Pear (Opuntia ficus indica) and Votre-Coach Alimantaire Cultivated in Adigrat, North of Ethiopia


Volume 10, Issue 1, October 2014, Pages 125–134

 Quantitative Determination of Sugar Levels in Natural Plants of Cactus Pear (Opuntia ficus indica) and Votre-Coach Alimantaire Cultivated in Adigrat, North of Ethiopia

D. Worku Batu and T. Wunesh Solomon

Original language: English

Received 1 August 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


In this work, a quantitative study for sugar concentration determination was conducted using Uv-Visible Spectroscopy. Glucose and Sucrose of three extracted plants fruit were analysis by refractometer and thin layer Chromatography before applied for quantitative determination. The TSS values recorded have a good agreement with the Sucrose standard and previously analyzed sugar in fruit and vegetables. TSS of Coach-fruit, Cactus Opuntia and Peel is 18Bix%, 18Brix% and 21Brix% respectively. These results have a good precision with the TSS sugar found in Apple species (18.83Brix %), Potato species (19.10%) and Grape (17.50Brix %) fruit-vegetables.The single spot observed on TLC, assay the purity of sample. Calibration models for Glucose and Sucrose determination were developed using the standard solution. Sugar concentrations were determined from single standard and calibration curve. The mean concentrations of Sucrose and Glucose in Coach-fruit, Cactus pear and Cactus peel, were 143.035mg/ml, 42.420mg/ml, and 0.943mg/ml for Sucrose; and 86.912mg/ml, 18.938mg/ml and 8.810mg/ml for Glucose determined.

Author Keywords: sugar, carbohydrate, glucose, sucrose, cactus pear.


How to Cite this Article


D. Worku Batu and T. Wunesh Solomon, “Quantitative Determination of Sugar Levels in Natural Plants of Cactus Pear (Opuntia ficus indica) and Votre-Coach Alimantaire Cultivated in Adigrat, North of Ethiopia,” International Journal of Innovation and Scientific Research, vol. 10, no. 1, pp. 125–134, October 2014.