Volume 9, Issue 1, September 2014, Pages 54–60
Sangha R. Bijekar1 and M.C. Gayatri2
1 Department of Molecular Biology, Bangalore University, Bangalore 560 056. Karnataka, India
2 Department of Botany Bangalore University, Bangalore 560 056. Karnataka, India
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In the present investigation, an attempt has been made to accelerate the flavonoid synthesis in Baliospermum montanum (Wild.) Muell-Arg., Dryptes roxburghii (Wall.) Huresawa and Codiaeum variegatum (L.) Bl, members of Euphorbiaceae family. Internodal explants were used to induce callus. Maximum growth of callus was observed in Baliospermum montanum and Codiaeum variegatum at 2mg/L and 3mg/L of 2,4-D respectively whereas Dryptes roxburghii showed maximum growth of callus at 3mg/L of BAP. Suspension culture was obtained from well developed callus. Three elicitors were used to induce flavonoid synthesis namely phenylalanine, copper sulphate and Pseudomonas aeruginosa. Among three, phenylalanine was found to be the best elicitor and maximum production of flavonoid was observed in Baliospermum montanum.
Author Keywords: Baliospermum montanum, Dryptes roxburghii, Codiaeum variegatum, callus, suspension culture, Elicitor and flavonoid.
Sangha R. Bijekar1 and M.C. Gayatri2
1 Department of Molecular Biology, Bangalore University, Bangalore 560 056. Karnataka, India
2 Department of Botany Bangalore University, Bangalore 560 056. Karnataka, India
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In the present investigation, an attempt has been made to accelerate the flavonoid synthesis in Baliospermum montanum (Wild.) Muell-Arg., Dryptes roxburghii (Wall.) Huresawa and Codiaeum variegatum (L.) Bl, members of Euphorbiaceae family. Internodal explants were used to induce callus. Maximum growth of callus was observed in Baliospermum montanum and Codiaeum variegatum at 2mg/L and 3mg/L of 2,4-D respectively whereas Dryptes roxburghii showed maximum growth of callus at 3mg/L of BAP. Suspension culture was obtained from well developed callus. Three elicitors were used to induce flavonoid synthesis namely phenylalanine, copper sulphate and Pseudomonas aeruginosa. Among three, phenylalanine was found to be the best elicitor and maximum production of flavonoid was observed in Baliospermum montanum.
Author Keywords: Baliospermum montanum, Dryptes roxburghii, Codiaeum variegatum, callus, suspension culture, Elicitor and flavonoid.
How to Cite this Article
Sangha R. Bijekar and M.C. Gayatri, “In vitro techniques to accelerate flavonoid synthesis in some Euphorbiaceae members,” International Journal of Innovation and Scientific Research, vol. 9, no. 1, pp. 54–60, September 2014.
