[ Profil électrophorétique oligoclonal à l’électrophorèse des protéines sériques dans la zone des gammaglobulines: A propos de deux cas ]
Volume 73, Issue 1, June 2024, Pages 72–77
Kawtar Lassouli1, Essayh Yousra2, Asmaa Morjan3, N. Kamal4, and A. Madani5
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
3 Laboratoire de biochimie, CHU Ibn Rochd, Casablanca, Morocco
4 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
5 Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco
Original language: French
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The oligoclonal profile of immunoglobulins is reflected by the presence of more than two narrow monoclonal peaks on serum protein electrophoresis, corresponding to the synthesis of several Immunoglobulin (Ig) idiotypes and isotypes by selection of a small number of plasma cells. The aim of this study is to elucidate the etiologies of the oligoclonal profile detected in the gamma globulin zone during the electrophoretic analysis of serum proteins of patients hospitalized and consulting at the Ibn Rochd University Hospital in Casablanca. This is a retrospective study spread over 8 months from January 1 to September 1, 2020, focused on the analysis of electrophoretic profiles of sera collected from hospitalized and consulting patients at CHU Ibn Rochd. The PES was carried out using the capillary electrophoretic technique on a capillarys automated system, and total protein determination by Biuret colorimetric method on an Architect automated system. Immunofixation was performed on agarose gel using the Hydrasys Sebia automated system, and immunofixation on capillarys 2 Flex piercing. Our study revealed 2 oligoclonal profiles: The first from a patient hospitalized in the nephrology department for nephrotic syndrome and renal failure, the results of his work-ups were as follows: serum protein immunosubtraction showed the presence of an IgG kappa immunoglobulin and an IgM lambda, and histopathological examination of the kidney showed glomerulonephritis. IgM lambda, and histopathological examination of the kidney showed glomerulonephritis stage 1. The second patient was admitted to the hematology clinic for anaemia and bone pain, His work-up was as follows: serum protein immunofixation showed IgA kappa immunoglobulin, and urine protein immunofixation showed free kappa/kappa light chain. Myelogram revealed multiple myeloma. The oligoclonal profile must be interpreted on a case-by-case basis according to the context of each patient, to reveal damage to the immune system and ensure immune system and ensure therapeutic follow-up.
Author Keywords: Oligoclonal, serum protein electrophoresis, immunofixation, gamma globulins.
Volume 73, Issue 1, June 2024, Pages 72–77
Kawtar Lassouli1, Essayh Yousra2, Asmaa Morjan3, N. Kamal4, and A. Madani5
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
3 Laboratoire de biochimie, CHU Ibn Rochd, Casablanca, Morocco
4 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
5 Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco
Original language: French
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The oligoclonal profile of immunoglobulins is reflected by the presence of more than two narrow monoclonal peaks on serum protein electrophoresis, corresponding to the synthesis of several Immunoglobulin (Ig) idiotypes and isotypes by selection of a small number of plasma cells. The aim of this study is to elucidate the etiologies of the oligoclonal profile detected in the gamma globulin zone during the electrophoretic analysis of serum proteins of patients hospitalized and consulting at the Ibn Rochd University Hospital in Casablanca. This is a retrospective study spread over 8 months from January 1 to September 1, 2020, focused on the analysis of electrophoretic profiles of sera collected from hospitalized and consulting patients at CHU Ibn Rochd. The PES was carried out using the capillary electrophoretic technique on a capillarys automated system, and total protein determination by Biuret colorimetric method on an Architect automated system. Immunofixation was performed on agarose gel using the Hydrasys Sebia automated system, and immunofixation on capillarys 2 Flex piercing. Our study revealed 2 oligoclonal profiles: The first from a patient hospitalized in the nephrology department for nephrotic syndrome and renal failure, the results of his work-ups were as follows: serum protein immunosubtraction showed the presence of an IgG kappa immunoglobulin and an IgM lambda, and histopathological examination of the kidney showed glomerulonephritis. IgM lambda, and histopathological examination of the kidney showed glomerulonephritis stage 1. The second patient was admitted to the hematology clinic for anaemia and bone pain, His work-up was as follows: serum protein immunofixation showed IgA kappa immunoglobulin, and urine protein immunofixation showed free kappa/kappa light chain. Myelogram revealed multiple myeloma. The oligoclonal profile must be interpreted on a case-by-case basis according to the context of each patient, to reveal damage to the immune system and ensure immune system and ensure therapeutic follow-up.
Author Keywords: Oligoclonal, serum protein electrophoresis, immunofixation, gamma globulins.
Abstract: (french)
Le profil oligoclonal des immunoglobulines se traduit par la présence de plus de deux pics monoclonaux étroits à l’électrophorèse des protéines sériques correspondant à la synthèse de plusieurs idiotypes et isotypes d’Immunoglobuline (Ig) par sélection d’un petit nombre de plasmocytes. L’objectif de cette étude est de d’élucider les étiologies du profil oligoclonal détecté dans la zone des gammaglobulines lors de l’analyse électrophorétique des protéines sériques des patients hospitalisés et consultants au centre hospitalier universitaire Ibn Rochd de Casablanca. Il s’agit d’une étude rétrospective étalée sur 8 mois allant du 1 er Janvier au 1 er Septembre 2020, portée sur l’analyse des profils électrophorétiques des sérums collectés des patients hospitalisés et consultants du CHU Ibn Rochd. L’EPS a été réalisée par la technique électrophorétique capillaire sur automate capillarys et le dosage des protéines totales par méthode colorimétrique de Biuret sur Automate Architect. Les immunofixations ont été réalisées sur gel d’agarose sur automate Hydrasys Sébia et l’immunofixation sur capillarys 2 Flex piercing. Notre étude a objectivé 2 profils oligoclonaux: Le premier d’un patient hospitalisé au service de néphrologie pour syndrome néphrotique et insuffisance rénale, les résultats de ses bilans étaient comme suit: l’immunosoustraction des protéines sériques a montré la présence d’une immunoglobuline de type IgG kappa et d’une IgM lambda, et l’examen histopathologique du rein a montré une glomérulonéphrite extramembraneuse stade1.Le deuxième patient hospitalisé en hématologie clinique pour anémie et douleurs osseuses, ses bilans étaient comme suit: l’immunofixation des protéines sériques a objectivé la présence d’une immunoglobuline de type IgA kappa, et l ‘immunofixation des protéines urinaires a montré la présence d’une chaine légère de type kappa/ kappa libre. Le myélogramme a révélé un myélome multiple. Le profil oligoclonal doit être interprété au cas par cas en fonction du contexte de chaque patient, en permettant de révéler une atteinte du système immunitaire et assurer le suivi thérapeutique.
Author Keywords: oligoclonal des immunoglobulines, l’analyse électrophorétique, l’immunofixation des protéines sériques, gammaglobuline.
How to Cite this Article
Kawtar Lassouli, Essayh Yousra, Asmaa Morjan, N. Kamal, and A. Madani, “Oligoclonal electrophoretic profile on serum protein electrophoresis in the gamma globulin zone: About two cases,” International Journal of Innovation and Scientific Research, vol. 73, no. 1, pp. 72–77, June 2024.
