[ Dosage par Chromatographie en phase liquide à haute performance avec détecteur UV (HPLC/UV) de la microcystine-LA présente dans trois eaux surfaces en Côte d’Ivoire (Lagune Aghien, Retenue d’eau Adzopé et Seuil d’Agboville) ]
Volume 70, Issue 1, December 2023, Pages 21–26
Rosine Djeha Yao1, Julien Kalpy Coulibaly2, Martin Kouassi Konan3, Éric Kouamé Yao4, Mathias Ahoutou Koffi5, and Mireille Dosso6
1 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
2 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
3 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
4 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
5 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
6 Laboratoire de Bactériologie- Virologie, Institut Pasteur, Côte d’Ivoire
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cyanobcateria blooms represent a major global concern due to certain toxin-producing species. These toxins are classifies into 3 groips bases on the mode of action: neurotoxins, dermatotoxins and hepatotoxins. Microcystins belonging on the group of hepatotoxins are the most studied, the most dangerous and the most encountered in waterways. It is in this context that we proposed to evaluate the microcystin content specifically LA which is as dangerous as the LR analogue. Monthly monitoring from june to september 2021 allowed us to collect water samples from three surface waters in Ivory Coast (Aghien Laoon, Adzopé Water Reservoir and Angboville Seuil). Microscopic observations made it possible to identify different cyanobacteria, and the determination of microcystin LA (MC-LA) present in the water samples was carried out by HPLC/UV. The results revealed the presence of fifteen (15) genera of cyanobacteria in the three water bodies studied including four (4) potentially producing microcystin. Oscillatoria sp was the most represented with a frequency àf 100% followed by genera Aphanocapsa, Calothrix, Chroococcus, Cylindrospermopsis Merismopedia, Microcystis, and Planktothrix (66,66%). The genera Anabaena, Anabaenopsis, Lyngbya, Phormidium, Pseudanabaena, Snowella and Synechococcus were the least represented (33.33%). The variant LA was identified on the Aghien lagoon and measured at different concentrations, the maximum and minimum values of which were respectively 3.5 µg/L at station 4 and 0.68 µg/L at station 1.
Author Keywords: Cyanobacteria, Microcystin LA, HPLC/UV, Ivorian Waters.
Volume 70, Issue 1, December 2023, Pages 21–26
Rosine Djeha Yao1, Julien Kalpy Coulibaly2, Martin Kouassi Konan3, Éric Kouamé Yao4, Mathias Ahoutou Koffi5, and Mireille Dosso6
1 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
2 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
3 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
4 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
5 Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Côte d’Ivoire
6 Laboratoire de Bactériologie- Virologie, Institut Pasteur, Côte d’Ivoire
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Cyanobcateria blooms represent a major global concern due to certain toxin-producing species. These toxins are classifies into 3 groips bases on the mode of action: neurotoxins, dermatotoxins and hepatotoxins. Microcystins belonging on the group of hepatotoxins are the most studied, the most dangerous and the most encountered in waterways. It is in this context that we proposed to evaluate the microcystin content specifically LA which is as dangerous as the LR analogue. Monthly monitoring from june to september 2021 allowed us to collect water samples from three surface waters in Ivory Coast (Aghien Laoon, Adzopé Water Reservoir and Angboville Seuil). Microscopic observations made it possible to identify different cyanobacteria, and the determination of microcystin LA (MC-LA) present in the water samples was carried out by HPLC/UV. The results revealed the presence of fifteen (15) genera of cyanobacteria in the three water bodies studied including four (4) potentially producing microcystin. Oscillatoria sp was the most represented with a frequency àf 100% followed by genera Aphanocapsa, Calothrix, Chroococcus, Cylindrospermopsis Merismopedia, Microcystis, and Planktothrix (66,66%). The genera Anabaena, Anabaenopsis, Lyngbya, Phormidium, Pseudanabaena, Snowella and Synechococcus were the least represented (33.33%). The variant LA was identified on the Aghien lagoon and measured at different concentrations, the maximum and minimum values of which were respectively 3.5 µg/L at station 4 and 0.68 µg/L at station 1.
Author Keywords: Cyanobacteria, Microcystin LA, HPLC/UV, Ivorian Waters.
Abstract: (french)
Les proliférations de cyanobactéries représentent une préoccupation mondiale majeure en raison de certaines espèces productrices de toxines. Ces toxines sont classées en 3 groupes en fonction du mode d’action: les neurotoxines, les dermatotoxines et les hépatotoxines. Les microcystines appartenant au groupe des hépatotoxines sont les plus étudiées, les plus dangereuses et les plus rencontrées dans les cours d’eaux. C’est dans ce cadre que nous nous sommes proposés d’évaluer la teneur en microcystine spécifiquement LA qui est autant dangereuse que l’analogue LR. Un suivi mensuel de juin à septembre 2021 nous a permis de collecter des échantillons d’eau sur trois cours d’eaux en Côte d’Ivoire (Lagune Aghien, Retenue d’eau d’Adzopé et Seuil d’Agboville). Les observations microscopiques ont permis d’identifier différentes cyanobactéries, et le dosage de la microcystine LA (MC-LA) présente dans les échantillons d’eau a été réalisé par HPLC/UV. Les résultats ont révélé la présence de quinze (15) genres de cyanobactéries dans les trois plans d’eau étudiés dont quatre (4) potentiellement producteurs de microcystine. Le genre Oscillatoria a été le plus représenté avec une fréquence de 100%, suivi des genres Aphanocapsa, Calothrix, Chroococcus, Cylindrospermopsis Merismopedia, Microcystis, et Planktothrix (66,66%). Les genres Anabaena, Anabaenopsis, Lyngbya, Phormidium, Pseudanabaena, Snowella et Synechococcus ont été les moins mis en évidence (33,33%). La variante LA a été identifiée sur la lagune Aghien et dosée à différentes concentrations dont les valeurs maximales et minimales ont été respectivement de 3,5 µg/L à la station 4.et de 0,68 µg/L à la station 1.
Author Keywords: Cyanobactéries, Microcystine LA, HPLC/UV, Eaux Ivoiriennes.
How to Cite this Article
Rosine Djeha Yao, Julien Kalpy Coulibaly, Martin Kouassi Konan, Éric Kouamé Yao, Mathias Ahoutou Koffi, and Mireille Dosso, “High-performance liquid chromatography with UV detector (HPLC/UV) of microcystin-LA present in three surface waters in Ivory Coast (Aghien Lagoon, Adzopé water Reservoir and Agboville Seuil),” International Journal of Innovation and Scientific Research, vol. 70, no. 1, pp. 21–26, December 2023.
