Volume 61, Issue 1, June 2022, Pages 43–49
Kodjo Djidjolé ETSE1, Komi ODAH2, Atsou Vincent AÏDAM3, Raoufou Aboudou RADJI4, and Akossiwoa Marie-Luce QUASHIE5
1 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
2 Laboratoire de Botanique et d’Écologie végétale, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
3 Laboratoire de Bio-Ingénierie Médico-environnementale, Centre Omnithérapeutique Africain (COA), 1ere rue COA, Zanguéra, BP 81718, Lomé, Togo
4 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
5 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
Original language: English
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Investigations have been carried out with the aim of producing secondary metabolites in vitro by cell cultures of Zanthoxylum zanthoxyloides. Cell dedifferentiation from explants was induced in the presence of 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid in combination with benzyladenine. HPLC analyzes revealed the uneven accumulation, in diversity and quantity, of a number of secondary metabolites in these cell cultures. These secondary metabolites are not synthesized by all cell strains with the exception of hesperidin which is present everywhere. Quantification of hesperidin and chelerythrine showed that 2,4-dichlorophenoxyacetic acid inhibits their synthesis unlike 1-naphthaleneacetic acid and light which seem to stimulate it. This study shows that it is possible to produce secondary compounds in vitro by cell cultures of Zanthoxylum zanthoxyloides.
Author Keywords: Zanthoxylum zanthoxyloides, cell culture, secondary metabolites, hesperidin, chelerythrine.
Kodjo Djidjolé ETSE1, Komi ODAH2, Atsou Vincent AÏDAM3, Raoufou Aboudou RADJI4, and Akossiwoa Marie-Luce QUASHIE5
1 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
2 Laboratoire de Botanique et d’Écologie végétale, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
3 Laboratoire de Bio-Ingénierie Médico-environnementale, Centre Omnithérapeutique Africain (COA), 1ere rue COA, Zanguéra, BP 81718, Lomé, Togo
4 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
5 Equipe Physiologie Horticulture Biotechnologie végétale, Laboratoire de Recherche Forestière, Faculté des Sciences, Université de Lomé, 01 BP 1515 Lomé 01, Togo
Original language: English
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Investigations have been carried out with the aim of producing secondary metabolites in vitro by cell cultures of Zanthoxylum zanthoxyloides. Cell dedifferentiation from explants was induced in the presence of 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid in combination with benzyladenine. HPLC analyzes revealed the uneven accumulation, in diversity and quantity, of a number of secondary metabolites in these cell cultures. These secondary metabolites are not synthesized by all cell strains with the exception of hesperidin which is present everywhere. Quantification of hesperidin and chelerythrine showed that 2,4-dichlorophenoxyacetic acid inhibits their synthesis unlike 1-naphthaleneacetic acid and light which seem to stimulate it. This study shows that it is possible to produce secondary compounds in vitro by cell cultures of Zanthoxylum zanthoxyloides.
Author Keywords: Zanthoxylum zanthoxyloides, cell culture, secondary metabolites, hesperidin, chelerythrine.
How to Cite this Article
Kodjo Djidjolé ETSE, Komi ODAH, Atsou Vincent AÏDAM, Raoufou Aboudou RADJI, and Akossiwoa Marie-Luce QUASHIE, “Synthesis and accumulation of secondary metabolites by cell cultures of Zanthoxylum zanthoxyloides Lam. (Zepernik and Timler): Quantification of hesperidin and chelerythrine,” International Journal of Innovation and Scientific Research, vol. 61, no. 1, pp. 43–49, June 2022.
