Volume 30, Issue 2, May 2017, Pages 242–250
Karima Selmaoui1, Amina Ouazzani Touhami2, Afifa Mouria3, Rachid Benkirane4, and Allal Douira5
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique, Biotechnologie et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of this study is to detect in vitro activity of pectinolytic enzymes (trans-eliminase polygalacturonase and pectin) and cellulolytic five fungal species responsible for rotting apples: Alternaria alternata (E and S), Penicillium sp. (P), Trichoderma sp. (Tr), Trichothecium roseum (Tc) and Fusarium avenaceum (F). Lyase activity petine of these fungi is low during the first days of pectin degradation process but increases with age of cultures. The most important activity was recorded in Alternata alternata (0,146 U) and Trichothecium roseum (0,100 U) followed by Trichoderma sp. (0,078 U). The lowest activity was observed in Penicillium sp. and Fusarium avenaceum. The estimated activity of polyganlagturonase is important during the first days of pectin degradation by fungi and decreases during this process. Alternata showed a very significant activity (0,1467U) compared to other fungi. However, the polygalacturonase activity is very lost in Trichothecium roseum (0,0012U) and the important cellulolytic activity was detected in Fusarium avenaceum (0,0384 U).
Author Keywords: Enzymatic activity, fungi, rot, apples, storage.
Karima Selmaoui1, Amina Ouazzani Touhami2, Afifa Mouria3, Rachid Benkirane4, and Allal Douira5
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique, Biotechnologie et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The aim of this study is to detect in vitro activity of pectinolytic enzymes (trans-eliminase polygalacturonase and pectin) and cellulolytic five fungal species responsible for rotting apples: Alternaria alternata (E and S), Penicillium sp. (P), Trichoderma sp. (Tr), Trichothecium roseum (Tc) and Fusarium avenaceum (F). Lyase activity petine of these fungi is low during the first days of pectin degradation process but increases with age of cultures. The most important activity was recorded in Alternata alternata (0,146 U) and Trichothecium roseum (0,100 U) followed by Trichoderma sp. (0,078 U). The lowest activity was observed in Penicillium sp. and Fusarium avenaceum. The estimated activity of polyganlagturonase is important during the first days of pectin degradation by fungi and decreases during this process. Alternata showed a very significant activity (0,1467U) compared to other fungi. However, the polygalacturonase activity is very lost in Trichothecium roseum (0,0012U) and the important cellulolytic activity was detected in Fusarium avenaceum (0,0384 U).
Author Keywords: Enzymatic activity, fungi, rot, apples, storage.
Abstract: (french)
L’objectif de cette étude est de détecter, in vitro, l’activité des enzymes pectinolytiques (pectine trans-éliminase et polygalacturonase) et cellulolytiques de cinq espèces fongiques, responsables des pourritures des pommes: Alternaria alternata (deux isolats E et S), Penicillium sp. (P), Trichoderma sp. (Tr), Trichothecium roseum (Tc) et Fusarium avenaceum (F). L’activité petine lyase de tous ces champignons est faible durant les premiers jours du processus de dégradation de la pectine mais augmente avec l’âge des cultures. L’activité la plus importante a été enregistrée chez Alternaria alternata (0,146 U) et Trichothecium roseum ( 0,100U) suivie par Trichoderma sp. (0,078U). L’activité la plus faible a été observée chez Penicillium sp. et Fusarium avenaceum. L’activité de la polyganlagturonase estimée est importante durant les premiers jours de dégradation de la pectine par les champignons étudiés et diminue durant ce processus. C’est Alternaria alternata qui a montré une activité très importante par rapport aux autres champignons (0,1467U). Par contre, l’activité de la PG estimée chez Trichothecium roseum est très faible (0,0012U). L’activité cellulolytique la plus importante a été détecté chez Fusarium avenaceum (0,0384U).
Author Keywords: activité enzymatique, champignons, pourriture, pommes, conservation.
How to Cite this Article
Karima Selmaoui, Amina Ouazzani Touhami, Afifa Mouria, Rachid Benkirane, and Allal Douira, “Détection de l’activité enzymatique pectinolytique et cellulolytique des champignons responsables de la pourriture des pommes en conservation,” International Journal of Innovation and Scientific Research, vol. 30, no. 2, pp. 242–250, May 2017.
