Volume 1, Issue 2, May 2014, Pages 116–126
Badia DOUHRI1, Hikmat DOUHRI2, Abdellah FARAH3, Mohamed IDAOMAR4, Nadia SKALI SENHAJI5, and Jamal ABRINI6
1 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
2 Applied Physical Chemistry Laboratory, Team of materials and interfacial systems, Department of Chemistry, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
3 National Institute of Medicinal and Aromatic Plants, University Sidi Mohamed Ben Abdellah, BP 159, Taounate principale, 34 000, Morocco
4 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
5 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
6 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Essential oil from the aerial parts of Lavandula multifida L. (Lamiaceae), used in the Moroccan traditional medicine, was extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). 34 constituents, representing 95.25 % of the total oil were identified. The major components at the species level were Carvacrol (47.62%), ?- bisabolene (9.01%), and Dodecyl Acrylate (8.37%), Linalol (7.42%), Menthone (4.98%), ?- Caryophyllene (3.34%), ?- Pinene (3.21%). Antibacterial activity of this oil was tested against human pathogenic bacteria: gram-negative and gram-positive bacteria by the agar diffusion method. The minimum inhibitory concentrations (MIC) of the oil were determined by the microdilution technique. The oil showed significant inhibitory activity against the bacteria, S. Aureus (14.330 ± 0.577 mm), B. subtilis (12.670± 0.577 mm), P. mirabilis (12± 1 mm), P. vulgaris (11.67± 0.577 mm), L. innocua (10.660 ± 0.577 mm), L. monocytogenes (8.667± 0.577 mm), E. coli CECT (9.667± 0.577 mm) and E. coli K12 (9.333 ± 0.577 mm). No inhibitory activity was observed against the bacterium Ps. aeruginosa.
Author Keywords: Lavandula multifida, Lamiaceae, Hydrodistillation, GC/MS Analysis, Essential oil, Antibacterial activity.
Badia DOUHRI1, Hikmat DOUHRI2, Abdellah FARAH3, Mohamed IDAOMAR4, Nadia SKALI SENHAJI5, and Jamal ABRINI6
1 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
2 Applied Physical Chemistry Laboratory, Team of materials and interfacial systems, Department of Chemistry, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
3 National Institute of Medicinal and Aromatic Plants, University Sidi Mohamed Ben Abdellah, BP 159, Taounate principale, 34 000, Morocco
4 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
5 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
6 Biology and health Laboratory, Team of biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Essential oil from the aerial parts of Lavandula multifida L. (Lamiaceae), used in the Moroccan traditional medicine, was extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). 34 constituents, representing 95.25 % of the total oil were identified. The major components at the species level were Carvacrol (47.62%), ?- bisabolene (9.01%), and Dodecyl Acrylate (8.37%), Linalol (7.42%), Menthone (4.98%), ?- Caryophyllene (3.34%), ?- Pinene (3.21%). Antibacterial activity of this oil was tested against human pathogenic bacteria: gram-negative and gram-positive bacteria by the agar diffusion method. The minimum inhibitory concentrations (MIC) of the oil were determined by the microdilution technique. The oil showed significant inhibitory activity against the bacteria, S. Aureus (14.330 ± 0.577 mm), B. subtilis (12.670± 0.577 mm), P. mirabilis (12± 1 mm), P. vulgaris (11.67± 0.577 mm), L. innocua (10.660 ± 0.577 mm), L. monocytogenes (8.667± 0.577 mm), E. coli CECT (9.667± 0.577 mm) and E. coli K12 (9.333 ± 0.577 mm). No inhibitory activity was observed against the bacterium Ps. aeruginosa.
Author Keywords: Lavandula multifida, Lamiaceae, Hydrodistillation, GC/MS Analysis, Essential oil, Antibacterial activity.
How to Cite this Article
Badia DOUHRI, Hikmat DOUHRI, Abdellah FARAH, Mohamed IDAOMAR, Nadia SKALI SENHAJI, and Jamal ABRINI, “Phytochemical Analysis and Antibacterial Activity of Essential Oil Of Lavandula multifida L.,” International Journal of Innovation and Scientific Research, vol. 1, no. 2, pp. 116–126, May 2014.
