Introduction: Microbiological control of the environment in intensive care units is an integral part of the prevention of nosocomial infections and the control of outbreaks involving multidrug-resistant bacteria (MDR). The infection prevention and control team is at the heart of this approach. Particular concerns are the maintenance of a safe and hygienic care environment, and the minimization of microbial contamination of surfaces and equipment. The objective of our work was to determine the team's approach to the management of environment-related infections in intensive care units, to determine the bacterial ecology of these units, to specify the most contaminated sites and to present preventive and corrective actions. Method: A quantitative and qualitative study of the microbiological control of the environment was carried out between January 2018 and December 2020 at the level of intensive care units (ICU), and described the pre, per and post-microbiological processes applied to the hospital environment. Results: Of 350 samples collected, 33% were positive. The overall distribution of multidrug-resistant bacteria (MDR) isolated showed a predominance of gram-negative bacilli (GNB). The hygienists were responsible for the development and implementation of an environmental risk prevention plan including surveillance, prevention, training and evaluation. Conclusion: Our results showed that equipment and surfaces in intensive care units were heavily contaminated with multi-resistant bacteria. The control of this risk must be implemented through a multimodal strategy, with the intervention of a team specialized in the field.