[ Profil bactériologique des kératites bactériennes au Centre Hospitalier Universitaire Hassan II de Fès ]
Volume 53, Issue 2, March 2021, Pages 132–136
Sara Kouara1, Mehdi Filali2, Ghita Yahyaoui3, and Mustapha Mahmoud4
1 Laboratoire de Bactériologie, Centre Hospitalier Universitaire Hassan II, Fès, Morocco
2 Laboratoire de Bactériologie, Centre Hospitalier Universitaire Hassan II, Fès, Morocco
3 Département de Microbiologie, Laboratoire Central d’Analyses Médicales, CHU Hassan II de Fès, Morocco
4 Département de Microbiologie, Laboratoire Central d’Analyses Médicales, CHU Hassan II de Fès, Morocco
Original language: French
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The objective of our study is to analyse the different bacteriological profiles and their antibiotic sensitivity patterns of bacterial keratites in our bacteriology laboratory at the Hassan II University Hospital in Fez.A retrospective study carried out at the bacteriology laboratory of the Hassan II University Hospital of Fez over a period of 3 years from 01/01/2018 to 31/10/2020 in patients diagnosed with infectious keratitis and who have benefited from a corneal sample + culture for bacteriological study. Demographic data, clinical characteristics, bacteriological data and antibiotic resistance and sensitivity were collected.Out of 218 corneal swabs, bacteria were isolated from 123 samples (56.4%). The most frequent isolates were coagulase-negative Staphylococci (SCN) (51.5%), Staphylococcus aureus (22.2%) and Pseudomonas aeruginosa (14.2%). All gram-positive isolates were susceptible to vancomycin, but methicillin resistance was found in 36.9% of CNS cases and no cases of MRSA were recorded. All gram-negative isolates were susceptible to ceftazidime, gentamicin, tobramycin and amikacin. Resistance to ciprofloxacin was observed in 20% of the gram-positive isolates.CNS, Staphylococcus aureus and Pseudomonas aeruginosa were the microorganisms most often responsible for bacterial keratitis. Dual therapy using vancomycin + ceftazidime should be considered for empirical treatment until culture and susceptibility results are available in order to adapt antibiotic therapy.
Author Keywords: Bacterial keratitis, antibiotic sensitivity, corneal culture.
Volume 53, Issue 2, March 2021, Pages 132–136
Sara Kouara1, Mehdi Filali2, Ghita Yahyaoui3, and Mustapha Mahmoud4
1 Laboratoire de Bactériologie, Centre Hospitalier Universitaire Hassan II, Fès, Morocco
2 Laboratoire de Bactériologie, Centre Hospitalier Universitaire Hassan II, Fès, Morocco
3 Département de Microbiologie, Laboratoire Central d’Analyses Médicales, CHU Hassan II de Fès, Morocco
4 Département de Microbiologie, Laboratoire Central d’Analyses Médicales, CHU Hassan II de Fès, Morocco
Original language: French
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of our study is to analyse the different bacteriological profiles and their antibiotic sensitivity patterns of bacterial keratites in our bacteriology laboratory at the Hassan II University Hospital in Fez.A retrospective study carried out at the bacteriology laboratory of the Hassan II University Hospital of Fez over a period of 3 years from 01/01/2018 to 31/10/2020 in patients diagnosed with infectious keratitis and who have benefited from a corneal sample + culture for bacteriological study. Demographic data, clinical characteristics, bacteriological data and antibiotic resistance and sensitivity were collected.Out of 218 corneal swabs, bacteria were isolated from 123 samples (56.4%). The most frequent isolates were coagulase-negative Staphylococci (SCN) (51.5%), Staphylococcus aureus (22.2%) and Pseudomonas aeruginosa (14.2%). All gram-positive isolates were susceptible to vancomycin, but methicillin resistance was found in 36.9% of CNS cases and no cases of MRSA were recorded. All gram-negative isolates were susceptible to ceftazidime, gentamicin, tobramycin and amikacin. Resistance to ciprofloxacin was observed in 20% of the gram-positive isolates.CNS, Staphylococcus aureus and Pseudomonas aeruginosa were the microorganisms most often responsible for bacterial keratitis. Dual therapy using vancomycin + ceftazidime should be considered for empirical treatment until culture and susceptibility results are available in order to adapt antibiotic therapy.
Author Keywords: Bacterial keratitis, antibiotic sensitivity, corneal culture.
Abstract: (french)
L’objectif de notre étude est d’analyser les différents profils bactériologiques et leurs schémas de sensibilité aux antibiotiques des kératites bactériennes au sein de notre laboratoire de bactériologie du CHU Hassan II de Fès.Une étude rétrospective menée au laboratoire de bactériologie du CHU Hassan II de Fès étalée sur une période de 3 ans du 01/01/2018 au 31/10/2020 chez des patients chez qui une kératite infectieuse a été diagnostiquée et qui ont bénéficié d’un prélèvement de cornée + culture pour étude bactériologique. Les données démographiques, les caractéristiques cliniques, les données bactériologiques et la résistance et la sensibilité aux antibiotiques ont été recueillis.Sur 218 prélèvements cornéens, des bactéries ont été isolées dans 123 échantillons (56,4%). Les isolats les plus fréquents étaient des Staphylocoques à coagulase négative (SCN) (51,5%), Staphylococcus aureus (22,2%) et Pseudomonas aeruginosa (14,2%). Tous les isolats gram-positifs étaient sensibles à la vancomycine, mais une résistance à la méthicilline a été trouvée dans 36,9% des cas de SNC et aucun cas de SARM n’a été enregistré. Tous les isolats gram-négatifs étaient sensibles à la ceftazidime, à la gentamicine, à la tobramycine et à l'amikacine. La résistance à la ciprofloxacine a été observée dans 20 % des isolats gram positifs.Le SNC, Staphylococcus aureus et Pseudomonas aeruginosa étaient les micro-organismes les plus souvent responsables de la kératite bactérienne. La bithérapie utilisant la vancomycine + ceftazidime doit être envisagée pour un traitement empirique jusqu'à ce que les résultats de la culture et de la sensibilité soient disponibles afin d’adapter l’antibiothérapie.
Author Keywords: Kératite bactérienne, sensibilité aux antibiotiques, culture cornéenne.
How to Cite this Article
Sara Kouara, Mehdi Filali, Ghita Yahyaoui, and Mustapha Mahmoud, “Bacteriological profile of bacterial keratitis at the Hassan II University Hospital of Fez,” International Journal of Innovation and Scientific Research, vol. 53, no. 2, pp. 132–136, March 2021.
